WP5. Biomarkers related to gut microbiota functions (P1 leader,P2,P3,P4; M1-M36)
Task 5.1 Gut microbiota composition
Task 5.2. SCFA production
Task 5.3. Breath volatile compounds as non-invasive biomarkers
Task 5.4. Profiling of bile acids
Task 5.5. Profiling of conjugated fatty acids
Deliverables (D) and Milestones (M) of WP5 with delivery date
D5.1 development and validation of breath volatile compounds as non-invasive biomarkers (M18)
D5.2 test meal for intervention studies (M6)
D5.3 SOPs for biological sample processing (M6)
D5.4 report on biomarkers related to gut microbiota functions for existing cohorts (M24)
D5.5 report on biomarkers related to gut microbiota functions for CHITIN-GLUCAN intervention studies (M33)
D5.6 report on correlative analyses (M27,M36)
M5.1 selection of biomarkers related to gut microbiota function (M30)
Risk: problem of method sensitivity for measurement of several VOC and/or metabolites.
Mitigation measure: restriction of the biomarkers based on reproducibility and sensitivity; change in the extraction procedures (for bile acids); change in detector (for chromatography analysis); change in immunoassay kits for peptides (hypersensitive)